Molecular identification of Pleurotus ostreatus strains from Patagonia and their ability to retain laccase activity at low temperature

Auteurs

  • Maximiliano Rugolo Centro de Investigación y Extensión Forestal Andino Patagónico
  • Francisco Kuhar
  • Maria Belén Pildain
  • Bernardo E. Lechner
  • Mario Rajchenberg

DOI :

https://doi.org/10.33885/sf.2019.49.1202

Résumé

Background: Pleurotus spp. are fungi characterized by their ability to biologically degrade lignocellulosic materials. In Argentina, 6 wild Pleurotus species are known, being P. pulmonarius and P. ostreatus the ones with the greatest biotechnological interest.

Objective: To determine the phylogenetic relationships of Pleurotus strains from Patagonia and their enzymatic capacity (laccase) at low temperatures.

Methods: A phylogenetic analysis of the ITS regions of 8 wild Pleurotus strains from Patagonia and Parana region in northeast and central Argentina was carried out. Laccase activity of the strains was quantified using dimethoxyphenol (DMP) as substrate, incubating at 30 and 10 ºC, with and without previous heat treatment at 80 ºC.

Results and conclusions: Phylogenetic analysis of the ITS region of Pleurotus strains showed that Patagonian strains belong to P. ostreatus (5) and non-Patagonian strains to P. pulmonarius (3). Laccase activity was no relationship between the retention capacity and the origin of the strains, with more than 50 % retention of activity at 10 ºC, therefore it viable their use in biotechnological processes that require low temperature conditions.

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Biographie de l'auteur

Maximiliano Rugolo, Centro de Investigación y Extensión Forestal Andino Patagónico

Dr. Ciencia Biológicas de la Universidad de Buenos Aires

Téc. responsable Laboratorio de producción de blanco de hongos comestibles del CIEFAP

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Publiée

2019-07-17

Comment citer

Rugolo, M., Kuhar, F., Pildain, M. B., Lechner, B. E., & Rajchenberg, M. (2019). Molecular identification of Pleurotus ostreatus strains from Patagonia and their ability to retain laccase activity at low temperature. Scientia Fungorum, 49, e1202. https://doi.org/10.33885/sf.2019.49.1202

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