Chitinolytic activity in cell-free extracts from mycelial cells of Mucor rouxii

Authors

  • Alicia Chagolla Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato. Guanajuato, Guanajuato, México
  • Mario Pedraza Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato. Guanajuato, Guanajuato, México
  • Everardo López Romero 1. Instituto de Investigacón en Biología Experimental, Facultad de Química, Universidad de Guanajuato. Guanajuato, Guanajuato, México 2. Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del lnstituto Politécnico Nacional. México, D.F., México

DOI:

https://doi.org/10.33885/sf.1987.3.704

Abstract

Chit inase activity was demonstrated in the cytosol , a mixed
mem brane fraction and in the cell wall fraction from mycelium of !L
~· In all cases , chitinolytic activity was much more effe ctive
against nascent chitin than against preformed chitin. Results
showed that loss of chitin biosynthesis upon incubation of a mixed
membrane fraction at 28" C was not due to ai increased
depolimerization of the nascent polymer by endogenous chitinases.
Incubation with digitonin extracted chitinase activity from cell
walls but not from the mixed membrane fraction. The physiological
implications of distribution of chitinase in different subcellular
fractions of mycelial cells of ~ ~ remains to be
established.

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How to Cite

Chagolla, A., Pedraza, M., & López Romero, E. (2016). Chitinolytic activity in cell-free extracts from mycelial cells of Mucor rouxii. Scientia Fungorum, 3(3), 283–292. https://doi.org/10.33885/sf.1987.3.704

Issue

No. 3 (1987): RMM núm. 3 1987

Section

Original papers

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