Immunological activity of mycelial and yeast antigens from different growth phases of Sporothrix schenckii
DOI:
https://doi.org/10.33885/sf.1986.3.659Abstract
Sporothríx schenckii metabolic antigens are of great valué in the diagnosis
and prognosis of human sporotrichosis. The biological activity of these molecules
may vary depending on the different strains and culture media used, antigen
extraction methods and fungal morphology. In order to observe the effects of S.
schenckii mycelial and yeast antigens from different growth phases on their
immunological activity, the present study was undertaken. Metabolic antigens
were obtained from culture médium filtrates from different fungal growth phases.
They were tested by immunodiffusion (ID) and counterimmunoelectrophoresis
(CIÉ) with sporotrichosis patients sera. Protein and carbohydrate concentrations
were determined in all antigens. The results showed a gradual increase of the
antigen's carbohydrate concentration during the logarithmic growth phase while
the protein concentration remained constant. The immunological reactivity
showed an increase in the number of precipitin bands with ID and CIÉ from
antigens obtained from the initial stationary phase. Cross reactions were found
only in ID with one histoplasmosis serum. The best results were observed with
antigens from the late logarithmic growth phase.
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