First report of Orbilia brochopaga isolated from red-ring-disease coconut palms in Colima, Mexico
Abstract
Background: Bursaphelenchus cocophilus causes red ring disease in coconut palms, which is the main sanitary constraint affecting coconut cultivation. The exploration of fungi that parasitize B. cocophilus is a viable strategy for its management.
Objective: To search for nematophagous fungi in coconut palms affected by red ring disease.
Methods: Tissue samples were collected from “Brazilian Green Dwarf” coconut palms showing symptoms of the disease. A nematode extraction technique was used, tissues were incubated in hot water (40 °C, 1 h). The remaining liquid was subsequently sieved and plated on water agar to isolate fungi. The fungi obtained were characterized morphologically and molecularly.
Results and Conclusions: One isolate of Orbilia brochopaga was obtained. This represents the first record in coconut palms in Colima and Mexico. The strain was deposited in the Colección de Hongos Entomopatógenos del Departamento de Control Biológico del Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria (SENASICA).
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