In vitro antagonism, ligninolytic activity and cell wall hydrolases in the interaction of Trichoderma species with Curvularia eragrostidis isolated from pineapple
DOI:
https://doi.org/10.33885/sf.2022.53.1430Abstract
Background: Curvularia eragrostidis is the causal agent of pineapple leaf spot disease. The search for biological control agents against C. eragrostidis is an activity of agricultural interest. Objectives: To evaluate the antagonistic capacity of Trichoderma spp. on C. eragrostidis and to determine the H2O2, laccase, chitinase and β-glucanase activities.
Method: A strain of C. eragrostidis and six strains of Trichoderma spp. (T-82, T-84, T-85, T-94, T-98, T-99) were co-cultured. Interaction types were determined. Laccase activity, H2O2 and total protein in an enzymatic extract from the confrontation were determined spectrophotometrically. Qualitative chitinase and β-glucanase activities in C. eragrostidis and Trichoderma sp. T-98 were measured.
Results and conclusions: Trichoderma sp. T-98 inhibited C. eragrostidis by 61.0% with a “partial replacement after a distant inhibition” type of interaction. C. eragrostidis produced laccase (0.027 U/mg of protein) and H2O2 (0.33 mg/L), which increased when it was confronted with Trichoderma sp. T-94 (Laccase=0.219 U/mg of protein) and Trichoderma sp. T-98 (H2O2=6.47 mg/L), respectively. Trichoderma sp. T-98 produced chitinases and β-glucanase, while C. eragrostidis only produced chitinases. The increase in H2O2 and laccase is associated with the defense of C. eragrostidis against Trichoderma spp.
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