Classical molecular techniques for differentiation of special forms of Fusarium oxysporium
DOI:
https://doi.org/10.33885/sf.2015.3.1141Keywords:
MP-PCR, RAMPnr, RAPD, pathogen, polymorphismAbstract
The characterization and differentiation of special forms of Fusarium oxysporum by analysis of DNA polymorphisms are more appropriate
than just considering morphological or physiological characteristics. Currently, they have developed sophisticated molecular techniques
to estimate genetic variation, differentiate or identify microorganisms, such as those based on second-generation sequencing, but
they still have high costs and are not accessible to many laboratories that require diagnose or differentiate a particular plant pathogen.
In this paper, three classical molecular techniques were compared: RAPD, MP-PCR and RAMPnr in order to differentiate isolates of F.
oxysporum f. sp. ciceris, F. oxysporum f. sp. lycopersici, F. oxysporum f. sp. radicis-lycopersici and F. oxysporum f. sp coffeae. These techniques
detected polymorphisms between the special forms of Fusarium and even between genotypes of F. o f. sp. lycopersici. The groups
represented in dendrograms indicate that F. oxysporum is very diverse and that special forms analyzed are genetically different. These
techniques provide a simple, fast and inexpensive routine procedure for the characterization and subsequent pathogen’s identification
Downloads
Downloads
How to Cite
Issue
Section
License
Copyright notice
Open access policy
The authors who publish in this journal accept the following conditions:
In accordance with copyright laws, Scientia Fungorum recognizes and respects the authors’ moral rights, as well as the ownership of property rights, which will be transferred to the journal for dissemination in open access. Scientia Fungorum does not charge for submission and processing of articles for publication.
All the texts published by Scientia Fungorum –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The authors can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Scientia Fungorum (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Scientia Fungorum.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a complementary file.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC-By-NC-SA 4.0).
