Trichoderma species in soil cultivated with Agave tequilana in Los Altos South region, Jalisco and assessment of their antagonistic capacity against Thielaviopsis paradoxa
DOI:
https://doi.org/10.33885/sf.2010.3.1066Keywords:
molecular Identification, morphology, taxonomy, biological controlAbstract
A survey was conducted to determine the species of Trichoderma from the soil of
commercial plantations of tequila agave (Agave tequilana) in the Los Altos Sur region located
in Jalisco State, Mexico in order to study them as biocontrol agents against one of the plant
pathogens that attack the tequila agave. Sixty four cultures of Trichoderma were identified
morphologically and some of them by DNA sequencing analyses of the internal transcribed
space (ITS), translation elongation factor 1 alpha (tef1), actin (act), and calmodulin (cal).
Morphologically, 60 isolates (93.7%) were identified as T. longibrachiatum and ITS sequence
data for 25 of these cultures confirmed their identity. Three cultures (4.6%) were identified as
T. harzianum and DNA sequences of ITS, tef1, act and cal confirmed the result. One culture
(1.5%) was identified morphologically and by ITS sequence data as T. reesei. Antagonism
against Thielaviopsis paradoxa was evaluated for several isolates of each species of
Trichoderma. Isolates of T. longibrachiatum, T. viridescens and T. reesei produced
significantly higher inhibition against Th. paradoxa than T. harzianum
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