Relationship between protease and chitinase activity and the virulence of Paecilomyces fumosoroseus in Trialeurodes vaporariorum (Hemiptera: Aleyrodidae)
DOI:
https://doi.org/10.33885/sf.2008.3.1033Keywords:
biological control, virulence factors, entomopathogenic fungusAbstract
The production of protease and chitinase activities of isolates of Paecilomyces
fumosoroseus and their virulence in whitefly nymphs were evaluated. Enzyme production
was tested in l8 fungal isolates, on synthetic liquid medium with colloidal shrimp cuticle and
remazol bright blue stained colloidal chitin as substrates for protease and chitinase activity,
respectively. The virulence bioassay was carried out in second stage whitefly (Trialeurodes
vaporariorum) nymphs in 9 monospore cultures of selected isolates. The virulence was
determined by the median lethal concentration (LC ) in whiteflies. Isolates EH-506/3 and 50
3 3 EH-511/3 with the highest virulence (LC of 1.1 x 10 and 6.9 x 10 , respectively) also showed 50
the highest protease activity (PU/mL of 39.8 and 14.9, respectively) at 120 h. Chitinases
reached maximum activities at the end (264 and 312 h) of the in vitro enzymatic assay. This is
the first time that an ample intraspecific variability in protease and chitinase activities as well
as in virulence has been reported in a representative sample of P. fumosoroseus strains from
Mexico, which suggest that protease activities could be used in the selection of candidates for
whiteflies biological control.
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