Using ITS sequences to assess intraspecific genetic relationships among geographically separated collections of the myxomycete Didymium squamulosum

Autores

  • Katherine E. Winsett Department of Biological Sciences, University of Arkansas. Arkansas, E.U.A.
  • Steven L. Stephenson Department of Biological Sciences, University of Arkansas. Arkansas, E.U.A.

DOI:

https://doi.org/10.33885/sf.2008.3.1024

Palavras-chave:

ITS, intraspecific variation

Resumo

An analysis of ITS sequences of Didymium squamulosum, a myxomycete regarded
as cosmopolitan, shows variation among geographically separated isolates. ITS 1 and 2 and
5.8S rDNA were amplified from specimens collected in widely scattered localities. The
sequences were analyzed by parsimony analysis and the resulting trees show separate,
moderate and well supported clades grouping some, but not all, of the geographic locations.
ITS is the first molecular marker examined for intraspecific variation in myxomycetes, and
although sequence analysis shows statistically significant differences among sequences from
the 14 specimens considered, the heterogeneity in ITS 1 and ITS 2 appears to be too great for
any more meaningful biogeographical conclusions. Variation in the 5.8S and the amount of
variation found in ITS 1 and 2 may signify that the isolates examined represent a complex of
sibling species, although this particular marker cannot be used to make that distinction in this
myxomycete.

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Winsett, K. E., & Stephenson, S. L. (2016). Using ITS sequences to assess intraspecific genetic relationships among geographically separated collections of the myxomycete Didymium squamulosum. Scientia Fungorum, 3(27), 59–65. https://doi.org/10.33885/sf.2008.3.1024

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