Murine macrophages phagocytosis of Histoplasma capsulatum yeasts opsonized with different IgG subclasses
DOI:
https://doi.org/10.33885/sf.1991.3.782Abstract
Internalization kinetics of opzonized yeasts with different
IgG subclasses obtained from infected mice and the participation
of several Fe'!' receptors (Fc'l'R) were studied. Yeast cells were
incubated 30 min at 37"C with enriched fractions of ant i Histoplasma
IgG1 , IgG2a, or IgG2b separated by protein ASepharose
chromatography. IgG from non infected mice was used as
control. Before trigger i ng internalization at 37"C, opsonized
ye asts were washed and adjusted to a 5 :1 rat.i:o in re lation to
phagocytic cells. A murine macrophage J774.2 cell line monolayer
was used and yeasts' attachment on the cultured monolayer was
performed for 1 h at 4 "C, to standardize and synchronize the
inocula ingestion . IgG and FcTR participation was determined
through a modified ELISA test, using a goat IgM anti- mouse IgG
peroxidase-conjugate t o evidence IgG opsonized yeasts on the
macrophages' surface at different times of phagocytosis. Results
showed a decrease in the optical density (O.D.) determined by
ELISA at 15- 60 min, followed by a recovery of O.D. during the
further phagocytic times , suggesting a disappearance of Fc'l'R
during these times due to their utilization or cointernalization
t ogether with opsonized yeasts, as we ll as a possible recycling
mechanism. I n relation to the different IgG subclasses used,
results support the highest opsonizing activity for IgG1 and
IgG2a and a probable FcTRII participation in the internalization
of opsonized yeasts.
Downloads
Downloads
How to Cite
Issue
Section
License
Copyright notice
Open access policy
The authors who publish in this journal accept the following conditions:
In accordance with copyright laws, Scientia Fungorum recognizes and respects the authors’ moral rights, as well as the ownership of property rights, which will be transferred to the journal for dissemination in open access. Scientia Fungorum does not charge for submission and processing of articles for publication.
All the texts published by Scientia Fungorum –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The authors can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Scientia Fungorum (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Scientia Fungorum.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a complementary file.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC-By-NC-SA 4.0).
