Properties of chitin syntheses present in 16S subunits from Mucor rouxii

Authors

  • Ricardo Hernández Delgadillo Departamento de Genética y Biología Molecular, CINVESTAV, Instituto Politécnico Nacional. México, D.F., México
  • José Ruiz Herrera 1. Departamento de Genética y Biología Molecular del CINVESTAV, Instituto Politécnico Nacional 2. Instituto de Investigación en Biologia Experimental, Facultad de Química, Universidad de Guanajuato. Guanajuato, Guanajuato, México

DOI:

https://doi.org/10.33885/sf.1989.3.738

Abstract

Chitin synthetase present in crude wall-membrane fractions from Mucor roux11
was dissociated with digitonin and separated in the form of 16S subun~y centrifugation
in lineal sucrose gradien~s. This enzyme synthesized microfibrils made of
highly crystalline -<-chitin. Chitin synthetase from 16S subunits was extremely unstable
specially once activated by controlled proteolysis. These results plus the
effect of inhibitors of neutral proteases on enzyme stability led to the concl ussion
that 16S subunits contain 3 forms of chitin synthetase in an active equilibrium:
pre·"zymogen", "zymogen" and active enzyme.
General characteristics of 16S-chitin synthetase are very similar to those of
the chitosomal enzyme. However, it was observed that whereas the properties of the
allosteric site of both enzymes were a~most identical, the catalytic site lost affinity
for the substrate, the cofactor Mg +, and the competitive inhibitor, polyoxin D.

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How to Cite

Hernández Delgadillo, R., & Ruiz Herrera, J. (2016). Properties of chitin syntheses present in 16S subunits from Mucor rouxii. Scientia Fungorum, 3(5), 23–41. https://doi.org/10.33885/sf.1989.3.738

Issue

No. 5 (1989): RMM núm. 5 1989

Section

Original papers

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