Genetic diversity of Neopestalotiopsis sp. in strawberry, evaluated using RAPD and mycelial
Abstract
Background: In Mexico, strawberry cultivation is affected by Neopestalotiopsis, which damages several plant organs and reduces yield. The variation in symptoms suggests a high genetic diversity of the pathogen, which makes studying its population structure necessary to improve its management.
Objective: To analyze the genetic variability of Neopestalotiopsis strains isolated from strawberry plants on three municipalities in the state of Michoacan, in order to provide information that contributes to the understanding of the pathogen's diversity and its possible relationship with the observed symptomatology.
Methodology: Seventeen Neopestalotiopsis strains isolated from strawberry in Michoacan were characterized morphologically and molecularly, describing their colony color, presence of acervuli, and conidial dimensions. Genetic variability was assessed using RAPD and ITS, a phylogenetic tree was constructed, and mycelial compatibility tests were used to complement the analysis.
Results and discussion: Morphological observations and RAPD analyses revealed considerable genetic variability, clustering the strains into five main groups. This diversity reflects the presence of close and differentiated lineages, suggesting a complex population structure and a possible association with the variety of symptoms observed in the field.
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