Production of blastospores of Simplicillium lanosoniveum in liquid culture media
DOI:
https://doi.org/10.33885/sf.2021.52.1392Abstract
Background: Fungi of Simplicillium genus are natural enemies of the coffee rust, Hemileia vaxtatrix. The production of high concentrations of mycoparasite propagules is essential for the biorational management of the pathogen.
Objectives: To evaluate the production of blastospores of Simplicillium lanosoniveum in vitro.
Methods: The production of blastospores of S. lanosoniveum (SL) was evaluated in four liquid culture media with different sources of carbon and nitrogen: base medium (MB), Jackson medium (MJ), Bayfolan-Soy medium (BS) and Bayfolan-Chitin medium (BQ), inoculated with a suspension of SL (1x106 conidia / 50 mL of medium).
Results and conclusions: The culture media BS and BQ registered the highest blastospore production 14 days after inoculation (BQ=1.2X108 and BS=4.8X107). These results demonstrate that their use is factible to reproduce S. lanosoniveum.
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