The system Cre/loxP as a tool in the molecular study of Yarrowia lipolytica
DOI:
https://doi.org/10.33885/sf.2011.3.1077Keywords:
heterologous expression, recombinase, genetic markerAbstract
The non-conventional yeast Yarrowia lipolytica has been broadly studied, its whole
genome sequence is known and public, and there are many molecular tools for study itself.
However, their genetic markers are limited. Therefore, we need to recycle these markers.
Taking advantage of Y. lipolytica has the ability for express different sources of recombinant
protein, we constructed one replicating plasmid for Cre1 recombinase from one replicative
plasmid under the direction of a strong promoter. The recombinase activity was evaluated in
mutants obtained by transposition. The mTnYl1plasmid includes two Lox at ends who are
target sequences of phage P1 recombinase. Our results indicated high efficiency and
specificity of the recombinase activity. Based on this strategy we replaced the URA3 selective
marker gene contained in mTnYl1 and then we sequenced the segments generated after
recombinase activity to corroborate its proper action. This strategy is simple as well as a
powerful molecular biology tool for Y. lipolytica study under selection and use of one single
marker
Downloads
Downloads
How to Cite
Issue
Section
License
Copyright notice
Open access policy
The authors who publish in this journal accept the following conditions:
In accordance with copyright laws, Scientia Fungorum recognizes and respects the authors’ moral rights, as well as the ownership of property rights, which will be transferred to the journal for dissemination in open access. Scientia Fungorum does not charge for submission and processing of articles for publication.
All the texts published by Scientia Fungorum –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The authors can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Scientia Fungorum (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Scientia Fungorum.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a complementary file.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC-By-NC-SA 4.0).
