Morphological analysis of Candida albicans biofilms produced at different pH and temperature conditions analyzed by optic and atomic force microscopy
DOI:
https://doi.org/10.33885/sf.2011.3.1075Keywords:
yeasts, growth in vitro, morphology of biofilms, microscopic techniquesAbstract
Microbial biofilm formation plays an important role in the cycle of life and
subsistence of many microorganisms. Candida albicans colonizes the surface of catheters,
prosthetic and epithelium forming biofilms that are extremely resistant to antifungal drugs. In
the present study, a strain of C. albicans was grown under different pH and temperature
conditions in order to analyze the morphology of biofilms formed by optical microscopy
(OM) and quantitatively by atomic force microscopy (AFM). Optical and atomic force
micrographs showed the formation of biofilms in all conditions tested. Main morphological
parameters of C. albicans biofilms (height, highlight and valleys) were determined by MFA,
showing heterogeneity in perimeter, area and volume values reached.
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