Mycelial growth of Lepista nuda native wild strains on culture media with different organic supplements
DOI:
https://doi.org/10.33885/sf.2008.3.1015Keywords:
Blewit, in vitro evaluation, coconut bagasse, sugarcane bagasse, oak powderAbstract
Mycelial growth of four strains of the wood blewit mushroom Lepista nuda on
culture media with different organic supplements (coconut bagasse, sugarcane bagasse, oak
sawdust), pH (6, 7, 8) and temperature (20°C, 25°C) was evaluated. Linear growth rate (K ) on t
agar medium was measured. Biomass production of mycelia and phenolic content on oak
liquid culture, under two different gaseous interchange treatments, culture bottles with filter
(F) and culture bottles without filter (WF), also were observed. Results showed a significant
influence of the supplements and strains on agar. The best growth responses were observed
-1 on oak agar medium at 20°C and 25°C (0.417 and 0.410 mm d , respectively). For both
temperatures, in all treatments no differences significantly were observed. The highest K by L. t
nuda strains was registered at pH 7. On liquid culture, the F treatment significantly increased
-1 the biomass production (4.10 mg mL ) by almost 1.8 times compared to the WF treatment
-1 (2.29 mg mL ) at 13 days of incubation. Phenols concentration decreased in both treatments
-1 but in F treatment the phenols concentration (0.79 mMol L ) decreased less than WF (0.62
-1 mMol L ). No correlation between the biomass production and phenolic content was
observed.
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