Minimal amount of aflatoxin b 1 to produce a mutation in the Ames test with salmonella thyphimurium Ta-98
DOI:
https://doi.org/10.33885/sf.2004.3.927Keywords:
Aflatoxins, mutagenesis, legal limitsAbstract
Different concentrations of aflatoxin B1 (A FB1) were tested in Salmonella
typhimurium TA 98 Ames test activated by rat liver microsomal S fraction and nicotinamide 9
adenine dinucleotide 3´phosphate (NADP) as cofactor, at ranges from 0.39 to 100 ng, with
an average of 20 spontaneous reversal colonies in the controls. The different AFB1
concentrations derived from a stock AFB standard solution of 1 μg/mL were quantified by 1
liquid chromatography (HPLC), with a Correlation coefficient of r= 0.99 in the calibration
curve. The minimum amount of AFB that can produce a mutation was 10 ng/g (=10 μg/mL). 1
This level can be useful to take into account for legal maximum tolerance levels in laws
applied to foods for human consumption. At this moment the maximum tolerance level used
for commercial transactions of NAFTA is of 20 ìg/kg. Nowadays legal maximum tolerance
limits are used for commercial transactions but do not protect human health. The
accumulative effect of AFB in DNA, as adducts, and the long periods of exposure to these 1
potent carcinogen makes its control more difficult in foods for human consumption.
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