Presence of Ochratoxin A (OTA), Citrinin and Fumonisin B, in breakfast cereals collected in French markets. Comparison of OTA analysis using or not immune affinity clean-up before HPLC
DOI:
https://doi.org/10.33885/sf.2004.3.920Keywords:
Ochratoxin A, Fumonisin, Citrinin, mycotoxins, Breakfast cerealAbstract
Cereals and other crops are susceptible to fungal attack either in the field or during
storage. The mycotoxins produced by these fungi are very stable and could be found in final
products such as breakfast cereal. The aim of this study was to evaluate the presence of
Fumonisin B (FB ), Ochratoxin A (OTA) and Citrinin (CIT) in some breakfast cereals collected 1 1
in French supermarkets. FB was purified by immunoaffinity columns and has been 1
detected, after derivatization, in 25/27 samples, ranging from 1 to 1110 μg/kg. FB has been 1
detected in samples containing corn, but also oat or rice. OTA and CIT recoveries were over
80 % by the conventional method of partition extraction, whereas the OTA recovery was
about 60% using immunoa finity clean-up in phosphate saline buffer. OTA has been detected
in 72.5% of the samples ranging from the limit of detection (0.1μg/kg) to 8.8 μg/kg. This
highest level was detected in a sample containing dry fruit and fibres. Seventeen percent of
the samples contained OTA above the EU limit of 3μg/kg. Twenty percent of them were also
contaminated by CIT.
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