Isolation, purification and chemical analysis of the sporangiophores of Phycomyces blakesleanus

Authors

  • Jorge Mundo Cansino Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional y Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, México, D. F.
  • José Ruiz Herrera Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional y Departamento de Genética y Biología Molecular, Centro de Investigación y Estudios Avanzados, Instituto Politécnico Nacional, México, D. F.

DOI:

https://doi.org/10.33885/sf.1979.2.491

Abstract

Cell walls from the sporangiophore oí Phycomyces blakesleanus were isolated
after mechanical breakage. Cell walls were purified by repeated washings with
NaCl and water. Chemical annlysis rcvealcd n complcx composition of the ccll
wall. Chitin, identified by X-ray diffraction, was the most abundant component.
Polyuronidcs and chitosan were present also in Inrge amounts. Chitosan was
identified by cytochemical reactions and by its solubility in acid. Cell walls contained
also proteins, lipids, phosphate and four neutral sugars: fucose, galactose,
glucose and mannose in'orden of abundante.

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How to Cite

Cansino, J. M., & Ruiz Herrera, J. (2016). Isolation, purification and chemical analysis of the sporangiophores of Phycomyces blakesleanus. Scientia Fungorum, 2(13), 39–45. https://doi.org/10.33885/sf.1979.2.491

Issue

No. 13 (1979): BSMM núm. 13 1979

Section

Original papers

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